ISHS Acta Horticulturae 744: I International Symposium on Human Health Effects of Fruits and Vegetables
THE EFFECT OF LEAF SPOT DISEASE ON SPECIFIC ANTIOXIDANT ACTIVITY OF STRAWBERRY |
| Authors: | B. Ehsani-Moghaddam, M.T. Charles, O. Carisse, S. Khanizadeh |
| Keywords: | antioxidant, electrophoresis, Mycosphaerella fragariae, reactive oxygen species |
| Abstract:
In controlled conditions, symptoms with varying degree of intensity depending on susceptibility of two strawberry cvs., i.e. resistant and susceptible, characterized as red spots were observed after thirty days of inoculation by Mycosphaerella fragariae pathogen. Inoculation resulted in increase in specific SOD activities in both cultivars, which higher level was observed in resistant. Comparison of the SODs isoform profiles in all samples derived from both resistant and susceptible cvs., by electrophoresis, indicated one constant sharp band identified as Mn-SOD, which in all samples derived from resistant cv. the intensity of the band was higher than that of susceptible. Another SOD isoform was also detected as CuZn-SOD, which was detected in all soluble proteins derived from resistant cv. This isoform was not observed in susceptible cv., however, when the amount of loaded protein was increased, it was illuminated as a faint band in sample derived from 3 days after inoculation, indicating insufficient production of CuZn-SOD isoform in susceptible cv. during oxidative burst induced by M. fragaria pathogen. Several bands were also observed in both cvs. containing both Fe and Mn as their co-factors (Fe,Mn-SOD). Unlike in resistant where the activity of Fe,Mn-SOD isoforms gradually and regularly increased and reached to the highest level in the third day of inoculation, in susceptible cv. the activity of the isoforms irregularly changed over 20 days of survey. | |
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