ISHS Acta Horticulturae 743: XXII International Eucarpia Symposium, Section Ornamentals, Breeding for Beauty - Part II
INTERSPECIFIC HYBRIDIZATION BETWEEN KALANCHOE BLOSSFELDIANA AND SEVERAL WILD KALANCHOE SPECIES WITH ORNAMENTAL VALUE |
| Authors: | Y. Izumikawa, I. Nakamura, M. Mii |
| Keywords: | pollination, ovule culture, flow cytometry, RAPD analysis, polyploidization, amphidiploid |
| Abstract:
Kalanchoe blossfeldiana (sect. Kalanchoe) is one of the most important ornamental indoor plants during the winter season. Breeding of K. blossfeldiana has been restricted to intra and interspecific hybridization with allied species. To enlarge the genetic variability, reciprocal crosses were carried out between K. blossfeldiana and several wild Kalanchoe species which belong to sect. Kalanchoe and sect. Bryophyllum, and ovule culture technique was applied to rescue the hybrid embryos. Hybridity of the F1 plants thus obtained was confirmed by flow cytometric and RAPD analyses. Consequently, 6 intra-section and 3 inter-section cross combinations were obtained in the present study. Reciprocal compatibility was observed in the crosses with 2 wild species. All the other hybrids could be obtained when K. blossfeldiana was used as maternal donor. Flower morphology and color of these hybrids were almost intermediate between both parental species. Since these F1 hybrids were sterile, amphidiploids of these hybrids were produced to restore the fertility by utilizing spontaneous chromosome doubling in tissue culture. | |
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