ISHS Acta Horticulturae 743: XXII International Eucarpia Symposium, Section Ornamentals, Breeding for Beauty - Part II
PRODUCTION OF TRANSGENIC PHALAENOPSIS PLANTS BY INTRODUCING GLUTATHIONE S-TRANSFERASE GENE INTO PROTOCORMS AT AN EARLY STAGE AFTER GERMINATION |
| Authors: | D.P. Chin, M. Mii, K.I. Mishiba |
| Keywords: | Agrobacterium tumefaciens, genetic transformation, orchid, over-expression, stress |
| Abstract:
Transgenic Phalaenopsis plants over-expressing glutathione S-transferase (GST) gene were produced by Agrobacterium-mediated transformation method. Protocorms at an early stage after germination were inoculated with A. tumefaciens strain EHA101 (pEKH35S163P) that harbors GST, hygromycin phosphotransferase and neomycin phosphotransferase II genes. A total of 68 independent transgenic plants derived from 6325 mature seeds were obtained 6 months after infection with Agrobacterium. Regenerated plants were grown in greenhouse and all plants bloomed within 2 years. Five months after pollination, 13 seed pods were obtained from 6 plants showing suitable commercial characters. Germinated Phalaenopsis T1 seedlings with GST gene were confirmed by PCR analysis. Northern blot analysis confirmed the expression of GST in hygromycin-resistant plantlets. | |
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