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ISHS Acta Horticulturae 739: II International Symposium on Saffron Biology and Technology

FACTORS INFLUENCING IN VITRO FLOWERING FROM STYLES OF SAFFRON

Authors:   Z. Jun, C. Xiaobin, C. Fang
Keywords:   Crocus sativus, explants age, growth regulator, style, temperature
Abstract:
This paper refers to the factors affecting in vitro flowering as follows in turn: the important factor was explants age according to style length, the second was culture condition containing temperature and light, the third was growth regulator. Style incised from 55-70 mm length floral buds with light blue perianths as explants cultured on media regulated with 26.8 µM 1-naphthaleneacetic acid and 31.1 µM 6-Benzylaminopurine in the dark at 20°C was the optimal condition for in vitro flowering induced, the induction ratio was 37.5-40.0%. As for growth regulator only, the higher 6-BA/NAA in the dark at 20°C was beneficial to in vitro flowering induced.

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