ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
SEROLOGICAL AND MOLECULAR VARIABILITY OF PLUM POX VIRUS IN TRANSGENIC AND CONVENTIONAL PLUMS |
| Authors: | I. Zagrai, M. Ravelonandro, R. Scorza, I. Gaboreanu, B. Ferencz, O. Popescu, D. Pamfil, L. Zagrai, A. Maxim |
| Keywords: | Plum pox virus, transgenic and conventional plum, serological and molecular variability |
| Abstract:
Plum pox or sharka is considered to be the most devastating disease of stone fruit, causing serious yield losses. Using a pathogen-derived resistance strategy as an alternative to conventional breeding, we produced a transgenic plum clone highly resistant to PPV. Environmental safety issues were evaluated related to the release of this virus-resistant transgenic crop. The objective of this study was to examine, under field conditions, the potential risk of a transgenic plum containing the PPV CP (coat protein) gene. These studies focused on the investigation of the diversity of virus populations by comparing the variability of PPV detected in the transgenic trees versus those found in conventional plums. Preliminary analysis based on serological and molecular data revealed that there was no significant difference between PPV isolated from transgenic and conventional plums. This study indicates that the release of the transgenic plums utilized in this study does not affect the diversity of indigenous PPV populations. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
