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| Authors: | A. Kalinina, D.C.W. Brown, M. Ravelonandro |
| Keywords: | PPV indicator plant, PPV strains, Sharka disease, virus indexing, virus infection |
Abstract:
Selected North American ornamental Prunus species such as P. glandulosa, P. tomentosa, P. cistena, P. americana, P. triloba, P. serrulata ‘Kwanzan’ and P. virginiana ‘Shubert’ were assayed for Plum Pox Virus (PPV) infection.
Two different trails of plants were used; in the former, one-year-old plants grafted on PPV susceptible ‘GF305’ rootstock and, in the second, self-rooted plants derived from tissue culture.
The four major PPV strains including D, M, EA and C were chip-budded to these plants.
Following symptom appearance, susceptible plants were confirmed by DAS ELISA and IC-RT-PCR. PPV was detected in most of Prunus species: P. glandulosa, P. tomentosa, P. cistena and P. americana. Some species such as P. serrulata ‘Kwanzan’, P. virginiana ‘Shubert’ and P. triloba were found to be tolerant to PPV. Based on the high susceptibility of P. glandulosa and P. tomentosa, these species may be candidates to use as an indicator of PPV infection.
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