ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
BEHAVIOUR OF AGROBACTERIUM RHIZOGENES-MEDIATED TRANSFORMANTS OF 'INMIL' (P. INCISA × SERRULA) CHERRY ROOTSTOCK |
| Authors: | PH. Druart, R. Gruselle |
| Keywords: | Prunus sp., genetic transformation |
| Abstract:
Several transformants have been regenerated on the roots of the most dwarfing cherry rootstock ‘Inmil’ (P. incisa × serrula) after treatment with Agrobacterium rhizogenes. Adventitious buds formed from initial hairy roots (‘RIB’ clones) and from the roots of primary somatic embryos (‘RMI 11’ clone). These clones differed by slight to severe alterations of their growth habit in vitro and ex vitro. All the plants rooted in presence of cytokinin during the multiplication stage, shoot elongation was weak with the ‘RMI 11’ clone more severely altered than ‘RIB’ clones but rooting was normal with all the regenerants. Differences between regeneration events have been noticed amongst ‘RIB’ clones after leaf rooting test performed in vitro. First responses of ‘RMI 11’ transformant to grafting and to further scion cutting performed with two wild cherry clones, showed improvement in grafting rate and rooting rate in absence of auxin treatment. All the lines declined after winter time when grown on their own roots in the greenhouse. That susceptibility to winter stress conditions may be a significant point. | |
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