ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
SEQUENCING AND ANNOTATION OF THE EVERGROWING LOCUS FROM WILD-TYPE AND MUTANT GENOMES REVEALS SEVERAL CANDIDATE GENES FOR THE CONTROL OF TERMINAL BUD FORMATION IN RESPONSE TO DORMANCY INDUCING CONDITIONS |
| Authors: | D.G. Bielenberg, S. Fan, G.L. Reighard, A.G. Abbott |
| Abstract:
‘Evergrowing’ (evg) peach is a naturally occurring mutant unable to cease terminal meristem growth or form terminal buds in response to winter dormancy-inducing conditions. The evg mutant is one of only two described natural mutants affecting winter dormancy in woody perennials. The evg mutation segregates as a recessive nuclear gene, and previous work fine mapped the trait to a 3.3 cM region between simple sequence repeat (SSR) markers. This region was physically mapped using a bacterial artificial chromosome (BAC) library, and a contig of three overlapping fragments was assembled. We completed the sequencing of a 132 kilobase region of the peach genome assembled from three overlapping BACs that encompass the complete EVG trait containing region. We present here 1) an analysis and annotation of this genomic region including putative and experimentally verified gene sequences; 2) a lambda phage library of an evg mutant genome, and screening of this library for inserts that span the mutant locus; 3) results of sequencing and analysis of the mutant locus and comparative expression studies of the genes directly affected by the mutation in wild-type and mutant trees. | |
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