ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
IDENTIFICATION OF RAPD MARKER LINKED TO RESISTANCE OF MUSA TO FUSARIUM OXYSPORUM |
| Authors: | A.Y. Zambrano, G. Martínez, Z. Gutiérrez, E. Manzanilla, L. Ruiz, L. Díaz, A. Contreras, A. Márquez, J.R. Demey |
| Keywords: | DNA marker, Fusarium wilt, resistance, RAPD |
| Abstract:
Banana and plantain (Musa spp.) are grown in more than 120 countries. They are cheap to produce, grow in a range of environments, and produce fruit year-round. Fusarium wilt of banana is widely regarded as one of the most destructive plant diseases. It is caused by soil-borne fungus Fusarium oxysporum f. sp. cubense. There is no chemical control available and the only way is to plant cultivars resistant/tolerant to the pathogen. In this study, we report a RAPD marker linked to susceptibility and resistance of Musa to F. oxysporum. Eight susceptible and ten resistant/tolerant accessions evaluated in field were used for this study. Genomic DNA was isolated from fully expanded young leaves. The RAPD patterns obtained with the primer OPK-03 detected a major fragment of 485 bp observed in the susceptible entrances, but absent in the resistant/tolerant entrances. The study of genetic relations between the genotypes susceptible and resistant/tolerant to F. oxysporum, using RAPD primers, defined the existence of an associated polymorphic pattern to the resistance. | |
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