ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
CHARACTERIZATION OF RIPENING-SPECIFIC MADS-BOX GENES FROM BANANA |
| Authors: | H. Friedman, E.T. Ocampo, T. Elitzur, E. Pesis, J.J. Giovannoni, J. Vrebalov |
| Keywords: | RIN, ethylene, postharvest, Musa |
| Abstract:
The study of developmental control of banana ripening has been initiated by cloning two MADS-box cDNA fragments (MaMADS1, MaMADS2) from ripening banana. The genes showed 66% homology to each other and 49 and 54% homology to LeRIN, respectively. Their homology to C-terminus is only 18 and 33%, respectively. MaMADS1 and MaMADS2 genes are different from several MADS-box genes isolated recently from various banana cultivars. None of the banana genes isolated so far exhibited high homology in their C-terminus to LeRIN. Typical climacteric ethylene production rate was observed nine days after harvest in the banana fruit. It preceded completion of yellow color development and a reduction in firmness. MaMADS1 and MaMADS2 exhibited high expression in ripe pulp. However, MaMADS1 was also expressed in other banana plant organs. Our results suggest that although MaMADS1 and MaMADS2 are phylogenetically distant from LeRIN, they may function in banana fruit ripening. | |
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