ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species

RESISTANCE TO PASSION FRUIT WOODINESS VIRUS IN TRANSGENIC PLANTS OF THE YELLOW PASSION FRUIT EXPRESSING THE VIRAL COAT PROTEIN GENE

Authors:   F. Trevisan, B.M.J. Mendes, S.C. Maciel, J.A.M. Rezende, M.L.C. Vieira, L.M.M. Meletti
Keywords:   Passiflora edulis, Potyvirus, disease control
Abstract:
We report here the use of the coat protein (CP) gene of Passion fruit woodiness virus (PWV), genus Potyvirus, to produce transgenic plants of passion fruit (Passiflora edulis f. flavicarpa). The CP gene of a severe PWV isolate from the State of São Paulo, Brazil (PWV-SP) was cloned into binary vectors pCambia 2300 and pCambia 2301, and introduced into Agrobacterium tumefaciens strain EHA 105. Transgenic plants from two cultivars, ‘IAC-275’ and ‘IAC-277’, were recovered. Integration of the PWV CP gene was confirmed in seven plant lines by Southern blot analysis, with variable number of copies. The 32 kDa PWV CP was detected by Western blot analysis in two plants. The results of three successive mechanical inoculations of the transgenic lines with three PWV isolates showed that the primary transformants T2 (‘IAC-277’) was immune to infection with all isolates. PWV-inoculated non-transformed plants exhibited severe mosaic symptoms 7 to 10 days after inoculation.
  • Full Text (PDF format, 134476 bytes)
  • Citation
  • Translate

Download Adobe Acrobat Reader (free software to read PDF files)

738_62     738     738_64

URL www.actahort.org      Hosted by KU Leuven      © ISHS