ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
CLONAL REGENERATION OF LITCHI (LITCHI CHINENSIS SONN.) VIA SOMATIC EMBRYOGENESIS |
| Authors: | S.H.T. Raharjo, R.E. Litz |
| Keywords: | Litchi chinensis, lychee, somatic embryo |
| Abstract:
Litchi embryogenic cultures were induced from leaflet explants in recent vegetative flushes of mature phase ‘Brewster’ trees on modified B5 medium containing 400 mg L-1 glutamine, 200 mg L-1 casein hydrolysate, 30 g L-1 sucrose, 3 g L-1 gellan gum, 4.52 μM 2,4-D and 9.30 μM kinetin. Embryogenic cultures were maintained on semi-solid MS medium supplemented with 4.52 μM 2,4-D and 0.91 μM zeatin at 26 ±1°C in darkness and with 4-5 week subculture intervals. Maturation of somatic embryos occurred in darkness on semi-solid MS medium without plant growth regulators, and fully enlarged somatic embryos germinated. Plantlets were maintained on 60 ml half-strength MS medium with 200 mg L-1 activated charcoal at 26 ±1°C under a 16h photoperiod provided by cool white fluorescent lights. Plants were successfully transferred to the greenhouse. | |
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