ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
GENETIC TRANSFORMATION OF APPLE WITH BIBAC VECTORS TO INCREASE SCAB RESISTANCE IN TRANSGENIC LINES |
| Authors: | J. Zhu, A. Zhou, S. Granozio, E. Andruccioli, E. Belfanti, M. Barbieri, B.A. Vinatzer, A. Patocchi, S. Tartarini, S. Sansavini |
| Keywords: | Malus × domestica, Venturia inaequalis, HcrVf genes |
| Abstract:
The apple scab resistance derived from Malus floribunda 821 is controlled by a single gene (named Vf). A positional cloning approach was used to identify candidate resistance genes by using a BAC library from cultivar ‘Florina’. At least three putative resistance genes, named HcrVfs, were identified at the Vf locus. Two BACs of the Vf region each containing the Hcrvf2 or Hcrvf4 genes were separately subcloned in a binary BIBAC vector for Agrobacterium-mediated transformation. Transgenic ‘Gala’ clones were acclimatized in greenhouse and molecularly characterized. A set of primers was designed to amplify specifically different T-DNA regions including the HcrVf genes by PCR. A scab resistance evaluation was also done. Deletions of different parts of the BIBAC vectors were observed in all the transgenic lines, mainly with regard to the HcrVf sequences. As expected by results of molecular analysis, all the clones were susceptible to scab. | |
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