ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
INDUCIBLE DNA PROMOTERS FOR USE IN APPLE |
| Authors: | J.L. Norelli, C. Bassett, T. Artlip, H.S. Aldwinckle, M. Malnoy, E.E. Borejsza-Wysocka, D. Gidoni, M. Flaishman |
| Keywords: | XVE, estradiol inducible, CAB, light inducible, transgenic, Gateway |
| Abstract:
A chemically inducible and a light inducible DNA promoter are being developed and evaluated for their ability to regulate gene expression in transgenic apple. The estradiol-induced XVE gene expression system was cloned into a binary vector (pBinPlusARS) compatible for use in Agrobacterium-mediated transformation of apple. To evaluate the estradiol-inducible binary vectors, a GUS marker gene was cloned into pBinPlusARS.XVE and pBinPlusARS.XVE.Gateway. When evaluated in tobacco under non-inducing conditions, GUS expression from the GUS containing XVE constructs was indistinguishable from that of an empty vector (no GUS coding region) negative control. However, in the presence of estradiol, GUS expression from the GUS containing XVE constructs was greater than that from a 35S promoter positive control. To evaluate the activity of the light inducible promoter of the peach chlorophyll a/b binding (CAB) protein and XVE in transgenic apple, binary vectors were constructed to allow comparison of test promoter activity with 35S activity. | |
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