ISHS Acta Horticulturae 738: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
MICROARRAY EXPRESSION PROFILING OF NAGAMI KUMQUAT IN RESPONSE TO CANKER |
| Authors: | A.A. Khalaf, G.A. Moore, F.G. Gmitter Jr. |
| Abstract:
Citrus canker disease, caused by a bacterial pathogen Xanthomonas axonopodis pv. citri, affects citrus species and citrus relatives in many areas of the world. Kumquats have apparent resistance to canker, the mechanism of which has not been determined. Forward and reverse subtractive cDNA libraries were constructed using Nagami kumquat mRNA to identify genes that are differentially expressed during the interaction with the canker pathogen. Some cDNA clones were selected for sequencing, and the sequences were analyzed using NCBI BLAST. Homologues to transcription factors, receptor and resistance genes known to be involved in plant-pathogen interaction were identified. cDNA microarrays containing 2304 of these genes were analyzed for expression profiles during the interaction with the canker pathogen. The results demonstrate how the use of microarray-based expression profiling methods can help elucidate plant pathogenesis-related response mechanisms and assign roles for previously uncharacterized genes. This can be considered to be a case study in citrus that has used these high throughput technologies to understand the defense mechanisms in Fortunella and citrus at the molecular level. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
