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ISHS Acta Horticulturae 736: III International Date Palm Conference

CHROMATOGRAPHIC SEPARATION OF FRUCTOSE FROM DATE SYRUP

Author:   S.M. Al Eid
Keywords:   ion chromatography
DOI:   10.17660/ActaHortic.2007.736.50
Abstract:
The objective of this study was to develop a process for separating fructose from a mixture of sugars containing essentially fructose and glucose that were obtained from date palm fruits. The extraction procedure of date syrup from fresh dates gave a yield of 86.5% solids after vacuum drying. To separate fructose from an aqueous solution of date syrup, solutions (20, 30 and 40 percent by weight) were introduced into a chromatographic column filled with Dowex polystyrene strong cation exchange gel matrix resin Ca2+ and divinylbenzen, functional group, sulfonic acid, particle size 320 micro-meter, with a flow rate of 0.025 and 0.05 BV/min., under 30oC and 70oC column temperature. After the date sugar solution batch, a calculated quantity of water was supplied into the column. Glucose was retained by the resin more weakly than fructose and proceeded faster into the water. Three fractions were collected: a glucose-rich fraction, return fraction, and fructose-rich fraction. The return fraction was based on when the peaks of fructose and glucose were reached, which could be determined by means of an analyzer (polarimeter) which was based on the property of glucose and fructose solutions to turn the polarization level of polarized light. A high yield of fructose was obtained at 70oC column temperature with flow rate of 0.025 BV/min and date syrup solution containing 40% sugar concentration. Because a low recovery was obtained using date syrup solutions having sugar concentrations of 20 and 30%, a 40% concentration was used. However, with the 40% date syrup, the average concentrations of glucose and fructose in the return fractions were increased, and could be used to dilute the thick date syrup solution.

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