|Authors: ||M.J. McCubbin, A. Zaid|
|Keywords: ||Phoenix dactylifera, off-types, in vitro|
Since first reports of off-types in date palm plants produced using micropropagation techniques, there has been much controversy regarding the process by which tissue cultured plants should be produced.
Using high concentrations of plant growth regulators in some embryogenesis protocols have been criticized, while organogenesis protocols have been criticized as being too lengthy and therefore less cost effective.
Using plant growth regulators in low concentrations during the initiation process may help to reduce possible variation.
Research done on initiation techniques showed that activated charcoal, and plant growth regulator type and concentration played a role in stimulating plant somatic changes.
Embryogenesis techniques for the second phase of multiplication are suggested.
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