ISHS Acta Horticulturae 735: I International Guava Symposium
MICROPROPAGATION OF GUAVA (PSIDIUM GUAJAVA L.) |
| Authors: | M. Mishra, R. Chandra, R. Pati, A. Bajpai |
| Keywords: | shoot bud culture, proliferation, MS media |
| Abstract:
A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. ‘Allahabad Safeda’. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth. Around 3 cm long shoots were excised and defoliated. The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent. The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water. The sterilized explants were cultured on MS medium supplemented with BAP (3.0 mg/L) for shoot bud induction and proliferation. The proliferated shoots were then subcultured on MS medium containing 10 mg/L IBA for rhizogenesis. The rooted plants were finally shifted to autoclaved coconut husk fortified with ˝ MS salt mixture for acclimatization. Acclimatized plantlets of guava cv. Allahabad Safeda were planted. | |
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