ISHS Acta Horticulturae 735: I International Guava Symposium
IN VITRO CALLUS INDUCTION IN GUAVA (PSIDIUM GUAJAVA L.) |
| Authors: | G. Singh, J.P. Tiwari, S. Lal |
| Keywords: | tissue culture, seedling explant, growth regulators, mercuric chloride, callusing, treatment duration |
| Abstract:
The investigation was carried out to study the response of explant and media composition on callus induction of guava seedlings cv. Pant Prabhat raised in vitro and ex vitro. Treatment of 0.1% HgCl2 for 10 minutes on ex vitro grown seedling was recorded maximum survival of explant after 5 days of inoculation. Maximum callus induction in shortest time (33.43%) from in vitro grown seedlings and ex vitro grown seedling (28.42%) were observed in MS media containing NAA (1 mg l-1) + 2,4-D (2 mg l-1) + Kinetin (1 mg l-1). Hypocotyl explant induced maximum callus in both types of explant irrespective of hormonal combinations. | |
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