ISHS Acta Horticulturae 734: VIII International Symposium on Plum and Prune Genetics, Breeding and Pomology
PLUM CULTIVAR DNA POLYMORPHISM GENERATED WITH RAPD AND ISSR MARKERS |
| Authors: | A. Lisek, M. Korbin, E. Rozpara, E. Żurawicz |
| Keywords: | cultivar diversity, molecular markers, Prunus domestica L. |
| Abstract:
Precise identification of fruit plant cultivars (based on genomic DNA analysis) is very important for germplasm collections, breeding and nursery practice. DNA markers for the differentiation of 15 plum (Prunus domestica L.) cultivars were selected. In total, 66 (RAPD) and 72 (ISSR) differentiating DNA fragments were obtained. Polymorphic DNA bands were observed in reactions with 34 out of 77 commercial RAPD primers (Operon Technologies Inc.) and 27 out of 31 ISSR primers (The University of British Columbia, Canada). The highest number of polymorphic products was generated in reactions with the primers OPB 07, OPB 12, OPG 10 and 824, 841, 855. The obtained results confirm the suitability of both techniques for identifying plum cultivars. | |
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