ISHS Acta Horticulturae 733: III International Grapevine Phylloxera Symposium
THE USE OF DNA MARKERS FOR PEST MANAGEMENT - CLONAL LINEAGES AND POPULATION BIOLOGY OF GRAPE PHYLLOXERA |
| Authors: | P.A. Umina, A.M. Corrie, K.S. Herbert, V.L. White, K.S. Powell, A.A. Hoffmann |
| Keywords: | Daktulosphaira vitifoliae, microsatellite, mtDNA, parthenogenesis, genetic diversity |
| Abstract:
Grape phylloxera, Daktulosphaira vitifoliae, is an important pest of grapevines, Vitis vinifera. The distribution and frequency of grape phylloxera genotypes varies within infested regions of Australia, suggesting the introduction of separate lineages of D. vitifoliae, which has important implications for the management of this pest. In this study, we apply six nuclear DNA microsatellite markers to characterize different phylloxera genotypic classes and present a comprehensive summary of the spatial distribution of D. vitifoliae genotypic lineages within Australian vineyards. Comparisons of mitochondrial DNA (cytochrome oxidase I) sequences are also performed, supporting previous conclusions that D. vitifoliae in Australia fall into two distinct clades. This information has generated important knowledge for the management of grape phylloxera and can be linked to historical information on introductions and outbreaks in Australia. | |
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