ISHS Acta Horticulturae 731: III International Symposium on Cucurbits
CLONING OF TRYPTOPHAN MONOXYGENASE GENE IN PSEUDOMONAS SYRINGAE AND TRANSFORMATION INTO CUCUMBER |
| Authors: | H. Liu, Z. Qin |
| Keywords: | iaaM gene, gene clone, vector construction, cucumber, transformation |
| Abstract:
Tryptophan monoxygenase iaaM gene was cloned from P. syringae pv. syringae 3023 by PCR. The results of nucleotide sequence determination showed that the complete nucleotide sequence was 1674 bp in length, and the GENBANK accession number was AY530536. The 2A12 promoter gene was cloned from tomato cultivar Dongnong 706 by using the same method. We constructed the expression vector pCAM-2AiaaT with the iaaM gene, and driven by fruit specific promoter 2A12. The iaaM gene was transferred into cucumber cultivar 649 mediated by Agrobacterium tumefaciens, and transgenic cucumber plants were confirmed by PCR and Southern blot. | |
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