ISHS Acta Horticulturae 730: VI International Symposium on Artichoke, Cardoon and Their Wild Relatives
VIRUS-FREE ARTICHOKE GERMPLASM: DIFFERENTIATION BETWEEN VIRUS-FREE AND CONTROL PLANTS DETECTED BY MOLECULAR MARKERS |
| Authors: | M.A. Pagnotta, N.A. Rey Munoz, M. Barba, F. Saccardo |
| Keywords: | globe artichoke, AFLP, ISSR, morphological |
| Abstract:
In central Italy global artichoke (Cynaria cardunculus var. scolymus) is mainly propagated vegetatively by offshoots, with and high risk of virus propagations and a consequent reduction of yield and quality production. Sanitary status of artichoke crop is seriously compromised by viral infections, which reduce yield and quality of production. For this reason, European regulations foresee the absence of the most dangerous pathogens, including Artichoke Latent Virus (ArLV), from the commercial propagated material. Some virus-free clones of global artichoke, obtained from meristem tip culture at ISPAVE, have been evaluated together with their virus-infected clones (control) at the ARSIAL’s experimental farm in Tarquinia (Viterbo, Italy). The RNA extracted, at the same phenological period, from 5 virus-free plants and from the correspondent 5 control clone plants, have been used to obtain C-DNA by RT-PCR, and perform differential analysis utilizing both ISSRs and AFLPs molecular markers. Even if the plants within groups are clones, so genetically uniform, different patterns were found within plants of the same clone. This could be probably due to a general high heterozygosity and phenotypic plasticity existing in the global artichoke. Despite that, comparing the two thesis (i.e. virus-free versus control plants) the results underline a general higher number of amplification bands in the control plants than from the virus-free plants; indicating a higher number of trascripted genes in the virus-infected plant than in the virus-free ones. Moreover, some differential band between virus-free and control plants have been found. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
