ISHS Acta Horticulturae 729: III Balkan Symposium on Vegetables and Potatoes
EFFECTS OF SILVER NITRATE, ACTIVATED CHARCOAL AND COLD TREATMENT ON THE IN VITRO ANDROGENESIS OF PEPPER (CAPSICUM ANNUUM L.) |
| Authors: | D. Özkum, R. Tıpırdamaz |
| Keywords: | anther culture, Solanaceae, AgNO3, cold pretreatment, embryo formation, haploid plants, culture media |
| Abstract:
The influence of cold-shock treatment of Capsicum annuum L. buds at 4°C for 48 h or 96 h, the additions of different concentrations of silver nitrate (AgNO3) (0, 1, 5 and 10 mg L-1) and 0.25% activated charcoal to the culture medium on pepper androgenesis were evaluated. Floral buds were collected whose anthers contained uninucleate microspores. Murashige and Skoog (MS) medium supplemented with combinations of 4 mg L-1 naphthalene acetic acid (NAA) + 1 mg L-1 benzyladenine (BA) or 1 mg L-1 NAA + 4 mg L-1 BA 0.8% (w/v) agar and 3% (w/v) sucrose. The highest numbers of embryos (12.5 embryos/100 anthers) were obtained from the control anthers which were not subjected to cold treatments and cultured on MS medium containing 4 mg L-1 NAA and 1 mg L-1 BA and activated charcoal without AgNO3. | |
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