ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
THE PROGRAMMED CELL DEATH, RESCUING CULTURE AND POLYPLOID PLANTLET ESTABLISHMENT IN ENDOSPERM CULTURE OF SPIDER LILIES (LYCORIS SPP.) |
| Authors: | L.S. Huang, P.C. Kuo, C.T. Shii |
| Keywords: | Lycoris, endosperm, apoptosis, regenerants, cytochimeras, DNA content |
| Abstract:
The endosperm tissue of spider lilies is initially destining to programmed cell death (PCD) during full cellularization stage about 32-35 days after pollination, manifested via Evanīs blue staining and accompanying genomic DNA degradation The occurrence of PCD is concomitantly lost of cell viability and proliferation capacity in vitro. The 25-32 days old of selfed- and crossed-endosperm was competent to produce 71.8% callusing on MS medium, and dramatically decreased to 32.5% and 2.0% by 30-35 and 30-40 DAP. The cultured immature endosperm may resume to PCD, but, can be totally arrested by addition of AgNO3 12 mg/L or 2,4-D 1-2 mg/L. The induced endosperm callus is composed of highly heterogenous cytochimeras varied in chromosome number or ploidies, and required serial subcultures for induction of somatic embryogenesis and organogenesis. The endosperm-derived plantlets were examined through cell flow cytometer revealed multiple genomic DNA peaks calculated as 1.37-1.47 times in relative DNA content of their parents. The self-pollinated endosperm regenerants of L. sprengeri and L. radiata 2n=22(22A) were cytologically examined as chimeric 28-34A chromosomes. The induced plantlets of L. aurea 2n=14(8M+6T) Ũ L. radiata were varied in the range 23=8M+6T+9A to 25=8M+6T+11A proved to be mixo-allotriploid. | |
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