ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
MICROPROPAGATION, DETECTION AND ELIMINATION OF DMV IN THE CZECH COLLECTION OF DAHLIA |
| Authors: | J. Sediva, P. Novak, J. Kanka, J. Laxa |
| Keywords: | Dahlia mosaic virus, Dahlia pinnata, meristem-tip culture, micropropagation, PCR methods, virus elimination |
| Abstract:
Production of virus free dahlia plants is necessary for the maintenance of the Czech collection of Dahlia pinnata. The effects of cytokinins (BA and zeatin) and time of sampling of primary explants were investigated. Higher regeneration rate of primary explants was observed when explants were grown on MS medium with 0.5 mg L-1 zeatin. Dahlia mosaic virus (DMV) could be eradicated from infected plants by meristem-tip culture. The effects of size of meristem tips on virus elimination and meristem regeneration were investigated. Apical meristems excised from in vitro culture of Dahlia were cultivated on MS medium with zeatin. Efficiency of meristem culture in elimination of DMV was 50% and 9% for plantlets developed from meristems in size 0.4-0.5 mm and 0.9-1 mm, respectively. Real-time PCR (polymerase chain reaction) and conventional PCR assay were used for detection of DMV. | |
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