ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
AN ALTERNATIVE PATHWAY FOR PLANT IN VITRO REGENERATION OF INSECTICIDAL TREE MELIA AZEDARACH L. DERIVED FROM THE INDUCTION OF SOMATIC EMBRYOGENESIS |
| Authors: | S. Sharry, W. Abedini, S. Lede, J.L. Cabrera Ponce, L. Herrera Estrella, R.M. Rangel Cano |
| Keywords: | Melia azedarach, somatic embryogenesis, direct and indirect morphogenesis, plant regeneration, adenine |
| Abstract:
A model for highly efficient method for plant regeneration from somatic embryos of chinaberry tree (Melia azedarach L.) was obtained. Mature seeds were cultured on Murashige and Skoog´s medium supplemented with NAA/BA to induce embryogenic tissues. Embryo proliferation occurred with high level citokinin concentration (10 mg/L BAP) and germination occurred on free hormone medium or in BAP/AG3 medium. Plants were regenerated from both, direct embryo formation and through an intermediary callus phase. Results showed 100% embryo conversion to plant. Regenerated plants were normal phenotypically and continued to grow after transfer to a greenhouse environment with 100% of surviving with high rate of growth. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
