ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO MORPHOGENESIS IN ZYGOTIC EMBRYO CULTURES OF CAROB TREE (CERATONIA SILIQUA L.) |
| Authors: | L. Custodio, A. Romano |
| Keywords: | conversion, regeneration, organogenesis, somatic embryogenesis, tissue culture |
| Abstract:
In this study, organogenic cultures were induced from zygotic embryos of carob tree. Basal medium consisted of Murashige & Tucker (1969), supplemented with 0.5 or 1 mg L-1 of 2,4-dichlorophenoxyacetic acid alone or combined with 0.5, 1, 2, 3 and 4 mg L-1 of kinetin. Calli initiation occurred in all the hormonal combinations tested. The zygotic embryo cultures differentiated two types of regenerants: somatic embryos (SEs) and shoots, with a maximum frequency of 30 and 100%, respectively. SEs exhibited morphological abnormalities, such as fusion and absence of cotyledons, and showed secondary embryogenesis. The regenerated shoots were rooted, hardened and transferred to the soil. | |
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