ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
INTERSPECIFIC HYBRIDIZATION IN GENUS FRAGARIA AND EVALUATION OF THE HYBRIDS FOR COLD HARDINESS AND RED STELE RESISTANCE USING IN VITRO METHODS |
| Authors: | R. Rugienius, D. Gelvonauskiene, I. Zalunskaite, J. Pakalniskyte, V. Stanys |
| Keywords: | strawberry, interspecific hybridization, cultivars, in vitro screening, cold hardiness, red stele resistance |
| Abstract:
For the hybridization 10 Fragaria species and forms with different genomic constitution and ploidy level were used. The highest berry set (55.8%) was in combinations where parental forms were octoploids: F. virginiana, F. chiloensis and F. ananassa. The highest output of fertile hybrids was in combinations where estimated ploidy level of seedlings was 2, 6 and 8x. The hybridization results were highly influenced by genomic constitution of parental forms. Depending on genomic constitution of parental forms berry set in diploid level varied between 0 and 42.7%, hybrid fertility between 56.5 and 89%. Utilizing the method of isolated embryos, it was possible to increase the output of seedlings up to 16-58%. For some combinations, it was the only method to receive seedlings. For screening of the hybrids for cold hardiness, we used freezing in vitro at -11°C. The viability of strawberry plants after freezing in vitro varied from 20.7% (‘Elsanta’) to 100% (seedlings F. orientalis x F. vesca and F. ananassa x F. virginiana). RAPD markers were used to identify Phytophthora fragariae resistance gene rpf1 in the interspecific hybrids. | |
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