ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
SOME ASPECTS OF EMBRYO DEVELOPMENT IN VITRO |
| Authors: | A. Pret´ova, B. Obert |
| Keywords: | embryocultures, somatic embryogenesis, androgenesis, flax, potato, barley, maize, pinus, oak, brassica |
| Abstract:
Embryogenesis in vitro involves embryocultures, somatic and gametic embryo formation (andro- and gynogenesis). On the basis of our findings, we can define phenomena and requirements common for both embryogenesis in situ and embryogenesis in vitro. Those can be summarized as follows: a) auxin plays a key role in embryogenesis – it seems that under influence of auxin the embryogenic determination of cells takes place, b) result of the polar auxin transport is a globular structure with full competency for correct organogenesis and the right polarity, c) right polarity is the basic requirement for an asymmetric cell division that is very probable a universal mechanism for realization of the embryogenic programme, d) right osmotic conditions have to be set in cultures, e) cells with an embryogenic competence have to be in a close physical contact. The embryogenesis in vitro ends up with formation of a bipolar structure – embryo – prepared to form a complete new plant body. | |
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