ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
CONSERVATIVE MICROPROPAGATION OF SOME ENDEMIC OR RARE SPECIES FROM THE DIANTHUS GENUS |
| Authors: | V. Cristea, M. Puscas, M. Miclaus, C. Deliu |
| Keywords: | in vitro, Dianthus, multiplication, rhysogenesis, photoautotrophic |
| Abstract:
The studies conducted on this topic, upon Dianthus genus, are justified both by its exceptional horticultural importance and by the fact that this genus has a great phytogeographic importance, because of the high rate of endemic species comprised in it. Also, many of the taxa included in this genus can be found on the national and European Red Lists. The in vitro biotechnology can successfully accomplish the needs for conservation of these species. 9 endemic or rare taxa from the Romanian and European Flora were studied. In vitro cultures were induced starting from seeds or caulinar, nodal and apical fragments. By successive transfers of apical and nodal explants on culture media having a varied hormonal balance (diverse combinations between NAA, IBA, IAA and BAP, K, 2iP, TDZ) a micropropagation of these species was achieved, with a maximum multiplication rate of 110 neoplantlets/explant (Dianthus petraeus ssp. simonkaianus). Ex vitro acclimatization was facilitated by an in vitro photoautotrophic phase, prior to the transfer in non-sterile conditions. At present, the studied species are acclimatized or they are in progress of being acclimatized in the “Alexandru Borza” Botanical Garden of Cluj-Napoca. | |
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