ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
A COMPARATIVE HISTOLOGICAL STUDY BETWEEN ´EMBRYOGENIC STRUCTURES´ AND ZYGOTIC EMBRYOS OF CLEMATIS HYBRIDS CULTIVATED IN VITRO |
| Authors: | L. Arène, M. Chevalier, N. Cognée, M. Tellier, A. Cadic |
| Keywords: | Clematis, histological study, somatic and zygotic embryos |
| Abstract:
Interspecific hybridizations have been made between herbaceous and climbing species of Clematis to obtain compact plants with big flowers. After achenes harvesting, embryos have been extracted and cultivated in vitro. This cultural method improves clearly the germination rate (+50%). Certain zygotic embryos give callus from which embryonic structures like somatic embryos appear. To confirm this, histological preparations of somatic and zygotic embryos have been made. The two types of embryos were embedded in technovit 7100 resin and prepared for microscopical observations as described by Chevalier et al. (1994). The so called embryonic structures have been confirmed as being somatic embryos. Cells and nucleus measurements in different parts of these two types of embryos indicate differences in cellular activity. Obtaining several somatic embryos from one zygotic embryo is interesting for the breeder since they may be a source of increased variability. This must be confirmed through the field observation of those plants. This opportunity might be also used as a facility for the development of mutagenesis. | |
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