ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO PLANT REGENERATION IN ZYGOTIC EMBRYO CULTURE OF IRIS REICHENBACHII |
| Authors: | S. Jevremonic, A. Subotic, LJ. Radojevic |
| Keywords: | in vitro culture, iris |
| Abstract:
Plant regeneration from mature zygotic embryo culture of Iris reichenbachii was studied. The effect of 2,4-dichlorophenoxyacetic acid (2,4-D, 0-10 mg L-1) on induction of morphogenesis in vitro is investigated. The best response for callus induction is observed on MS medium with 10 mg L-1 2,4-D. White embryogenic, yellow non-embryogenic and green organogenic calli are observed. The fresh weight, as well as, percentage portion of embryogenic and organogenic calli is 2,4-D dependent. Shoots are succesfuly multiplied by adventitive organogenesis from organogenic callus. Germination of somatic embryos and rooting of shoots were achieved on MS solid medium without hormones. | |
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