ISHS Acta Horticulturae 725: V International Symposium on In Vitro Culture and Horticultural Breeding
INCREASE IN THE QUALITY OF RASPBERRY CULTURES UNDER THE INFLUENCE OF FEEDDHA |
| Authors: | M. Zawadzka, T. Orlikowska |
| Keywords: | chlorosis, iron unavailability, micropropagation, adventitious regeneration, raspberry |
| Abstract:
The quality of raspberry shoot cultures was far from optimal because of leaf chlorosis increasing with the duration of culture. We, therefore, added iron in the form of the chelate FeEDDHA (50 mg/L) to the multiplication medium from which leaves for adventitious regeneration were collected or to the both – multiplication and regeneration medium. The FeEDDHA supplementation of multiplication medium eliminated leaf chlorosis and increased the number of adventitious shoots in three cultivars – ‘Beskid’, ‘Canby’ and ‘Veten’. The supplementation of regeneration medium increased the number of regenerating leaves of ‘Canby’, ‘Malling Seedling’ and ‘Norna’ and the number of the adventitious shoots of ‘Veten’. The highest level of regeneration was observed on the regeneration medium containing 78.8 mg/L FeEDDHA as the sole source of iron. | |
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