ISHS Acta Horticulturae 723: I International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation
OPTIMISATION OF IN VITRO CULTURAL CONDITIONS OF SOME OFFICINAL SPECIES |
| Authors: | M. Lucchesini, S. Pacifi, F. Tognoni, A. Mensuali-Sodi, G. Serra |
| Keywords: | Ajuga reptans L., environmental conditions, Lavandula officinalis Chaix x Lavandula latifolia Villars, Mentha piperita L., tissue culture. |
| Abstract:
In this work in vitro culture conditions for three species belonging to the Labiatae family were studied. In particular, a micropropagation protocol of Lavandino cv Grosso (Lavandula officinalis Chaix x Lavandula latifolia Villars) was developed. As regards this species it was verified that the hyperhydricity symptoms of axillary shoots were related to the agar concentration in the culture medium. Moreover, a reproducible in vitro propagation procedure was established for Mentha piperita L.. The results demonstrated that the method of supplying cytokinins affected the proliferation percentage. Moreover, studies concerning the influence of gravity on shoot formation showed that the perturbation of the explant orientation modified BA absorption inducing shoot hyperhydricity. Explants from wild plants of Ajuga reptans L. were micropropagated giving the source of material to start callus production: different callus proliferation and qualities were observed in dark and light conditions. The results from the experiments on the three species suggest that the interaction among physical- chemical factors may affect tissue cultured plants and it might often mask or give unexpected results generating mistakes in data evaluation. | |
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