ISHS Acta Horticulturae 722: XI International Symposium on Virus Diseases of Ornamental Plants
MOLECULAR BIOLOGY APPROACH FOR IMPROVING CHRYSANTHEMUM RESISTANCE TO VIRUS B |
| Authors: | S. Mitiouchkina, S.V. Dolgov, K. Zavriev, P.N. Kharchenko |
| Keywords: | Chrysanthemum morifolium Ramat., genetic transformation, virus B, coat protein |
| Abstract:
Chrysanthemum virus B (CVB) is an aphid-transmitted member of the carlavirus group, family Flexiviridae, occurring worldwide in cultivated varieties of chrysanthemum. The nucleotide sequence of the open reading frame 5 encoding the CVB coat protein was inserted in varied orientation into expression vectors for genetic engineering to improve chrysanthemum resistance to CVB infection. We obtained 12 transgenic chrysanthemum lines with antisense cDNA sequence, 5 lines with sense cDNA sequence, and 1 line with double sense cDNA sequence. The presence of the CVB coat protein gene in the plants was proved by PCR. | |
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