ISHS Acta Horticulturae 722: XI International Symposium on Virus Diseases of Ornamental Plants
GENETIC ENGINEERING OF POINSETTIA WITH THE AIM OF ENHANCING ITS RESISTANCE TO POINSETTIA MOSAIC VIRUS |
| Authors: | J.L. Clarke, S.S. Klemsdal, E. Floistad, A.K. Hvoslef-Eide, S. Haugslien, R. Moe, D.R. Blystad |
| Abstract:
Poinsettia (Euphorbia pulcherrima) is a major ornamental pot plant in many countries. Two viruses, poinsettia mosaic virus (PnMV) and poinsettia cryptic virus, often infect modern poinsettia cultivars. To produce virus-resistant poinsettia plants, genetic engineering has been considered. Since there is no established method available for the transformation of poinsettia, we have chosen an electrophoresis-based transformation method to generate poinsettia transformants. The main advantage of this method is that we can avoid the time-consuming tissue culture and regeneration procedure. To develop a reliable transformation method, the green fluorescent protein (GFP) gene has been used as a reporter gene. Here we report our preliminary results. | |
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