ISHS Acta Horticulturae 717: XIII International Symposium on Apricot Breeding and Culture
REGENERATION OF TRANSFORMED APRICOT PLANTS FROM LEAVES OF A COMMERCIAL CULTIVAR |
| Authors: | C. Petri, S. Lopez-Noguera, N. Alburquerque, L. Burgos |
| Keywords: | gfp, nptII, P. armeniaca |
| Abstract:
Efficient regeneration and transformation procedures have been developed with mature tissues from apricot cultivars. Integration of transgenes (gfp and nptII marker genes) in the plant cells have been demonstrated with transformed callus tissues by means of observation of gfp expression, exposure to high kanamycin doses, PCR and Southern blot hybridisation. However, production of transformed plants from commercial cultivars was difficult and only after the development of a progressive selection strategy have transformed apricot plants been obtained. A low selection pressure applied during the first days after co-culture, followed by a higher selection pressure afterwards, greatly improved transformation and selection efficiencies. Survival of transformed buds was affected by the selection pressure applied during recovery and elongation. Putative transformed shoots were multiplied in proliferation medium and then rooted to confirm gfp expression in the roots. DNA was extracted from proliferated shoots, for each putative transformed line, and checked by PCR and dot-blot hybridisation. | |
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