ISHS Acta Horticulturae 717: XIII International Symposium on Apricot Breeding and Culture
SELF-(IN)COMPATIBILITY IN PRUNUS ARMENIACA L.: ANALYSIS OF THE S-LOCUS STRUCTURE AND IDENTIFICATION OF S-HAPLOTYPE SPECIFIC S-RNASE |
| Authors: | S. Vilanova, C. Romero, L. Burgos, J. Martinez-Calvo, M. Vicente, G. Llacer, M.L. Badenes |
| Keywords: | apricot, genotyping, S alleles, gametophytic self-incompatibility |
| Abstract:
The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locus: an S-RNase and a recently-described, pollen-expressed, S-haplotype-specific F-box gene (SFB). Fragments containing genes homologous to the S-RNase and F-box genes in Prunus species have been described. In apricot (Prunus armeniaca L.), these genes were identified recently. Three identified S-RNase alleles and the F-box allelic variants (SFB1, SFB2 and SFB4) allowed elucidation of the S-locus structure in apricot. In this paper, from the analysis of the S-locus, we carried out the molecular typing of several S-alleles in apricot. A primer pair developed from conserved regions identified in the apricot S-locus was used to amplify the first S-RNase intron. Fragments containing the second intron were obtained using two sets of primers designed from published Prunus avium S-cDNA sequences. These results confirm genotypes established by other methods and establish five self-(in)compatibility groups in apricot. | |
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