ISHS Acta Horticulturae 716: VII International Protea Research Symposium
THE SEARCH FOR SEX-LINKED DNA MARKERS AND THE CONSTRUCTION OF PHYLOGENETIC RELATIONSHIPS AMONG SELECTED LEUCADENDRON SPECIES |
| Authors: | G. Yan, F. Shan, B. Croxford, R. Sedgley |
| Keywords: | dioecism, molecular genetics, breeding, RAPD |
| Abstract:
All Leucadendron species described so far are strictly dioecious. Our earlier studies suggested a single gene controlling system as most families segregated in a 1:1 ratio. We used bulk-segregate DNA analysis to search for DNA markers linked to the sex determining gene(s). Sixty-six genotypes from 11 species were examined using 45 RAPD primers. Universal DNA markers across all species were not identified. However, 12 sex-linked DNA markers were identified in individual species L. laureolum, L. procerum, L. discolor and L. salignum. At least four markers were further verified using three individual males and three individual females. More samples were taken to check the reliability of the markers before they can be used in the breeding program. As part of the molecular research, phylogenetic relationships among 11 Leucadendron species were also constructed based on the DNA markers. The molecular relationship between species is generally consistent with the taxonomy of the genus and is also in agreement with our hybridisation result – the closer the relationship, the easier to cross between them. | |
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