ISHS Acta Horticulturae 715: VIII International Symposium on Vaccinium Culture
CROSS-SPECIES AMPLIFICATION OF SSRS IN THE GENUS VACCINIUM |
| Authors: | P.S. Boches, K.E. Hummer, N.V. Bassil, J. Rowland |
| Keywords: | blueberry, genetics, DNA, microsatellites, EST |
| Abstract:
Primers were designed to amplify 36 simple sequence repeat (SSR) sequences identified in an Expressed Sequence Tag (EST) library derived from Vaccinium corymbosum. They were tested for amplification in other species of the genus Vaccinium. Two representatives each from the species V. ovatum, V. arboreum, V. elliottii, V. tenellum, V. darrowii, V. bracteatum, V. oldhamii (one representative only), V. macrocarpon, V. vitis-idea, V. parvifolium, V. crassifolium and diploid V. corymbosum were tested. Sixteen SSRs were amplified in all genotypes tested. Analysis of variance indicated significant differences between species in the number of loci that were amplified. EST-SSR loci developed in tetraploid V. corymbosum were most easily transferable to other members of section (§) Cyanococcus and least easily transferable to sections Oxycoccos, Herpothamnus, Myrtillus, and Batodendron in descending order. Several loci amplified exclusively in sections Cyanoccus, Batodendron, Bracteatum and Ciliata, possibly indicating a genetic link between Vaccinium in the southeastern United States and those present in Asia. | |
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