ISHS Acta Horticulturae 714: XXII International Eucarpia Symposium, Section Ornamentals, Breeding for Beauty
MOLECULAR BIOLOGY APPROACH FOR IMPROVING CHRYSANTHEMUM RESISTANCE TO VIRUS B |
| Authors: | T.S. Skachkova, T.Y. Mitiouchkina, S.A. Taran, S.V. Dolgov |
| Keywords: | Chrysanthemum morifolium Ramat., virus resistance, coat protein, genetic transformation, chrysanthemum virus B, antisense RNA |
| Abstract:
The chrysanthemum have been vegetatively propagated for a long time so the viruses and viroids which infect this group, have also been perpetuated. Chrysanthemum virus B (CVB) is a member of the carlavirus group and it is widespread throughout the world in cultivated varieties of chrysanthemum. Three expression vectors were constructed for Agrobacterium-mediated transformation of chrysanthemum (Chrysanthemum morifolium Ramat cv. ‘White Snowdon’) with the aim of improving the resistance to CVB infection. These constructs contained the double 35S promoter, the kanamycin-resistance selectable marker gene and the nucleotide sequence of the open reading frame 5 (ORF5) encoding the CVB coat protein in sense, antisense and double sense orientation. We obtained 12 transgenic chrysanthemum lines with antisense cDNA sequence, 7 lines with sense cDNA sequence and 20 lines with double sense cDNA sequence. The integration of the coat protein gene in the plants was detected by PCR. | |
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