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ISHS Acta Horticulturae 714: XXII International Eucarpia Symposium, Section Ornamentals, Breeding for Beauty

USE OF HOMOZYGOSITY IN THE RANUNCULUS ASIATICUS BREEDING PROCESS

Authors:   F. Ferrero, A. Duclos, L. Ottenwaëlder, M.J. Thiebaut, Y. Jacob
Keywords:   Ranunculus asiaticus, vitroculture, androgenesis, homozygosity, F1 hybrids
Abstract:
The in vitro androgenesis method is mainly goaled to produce homozygous diploid genotypes (HD) of Ranunculus asiaticus originated from cultivated heterozygous diploid genotypes. This presentation shows four important uses of the homozygosity in the ranunculus breeding process. 1) The fundamental use of in vitro androgenesis to produce homozygous HD progenitors. 2) The realization of crossing designs in order to increase the level of ability for producing HD plants directly from pollen grains. 3) The use of HD as intermediate contributors in crossing design between F1 hybrids (from combinations HD X HD) and inefficient genotypes, to increase the haploid induction ability in the progeny. 4) The use of androgenesis to increase HD male fertility which pointed out some limits of the method. In conclusion, this work is globally aimed to the production of high value F1 hybrids suitable for growers of ranunculus varieties.

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