ISHS Acta Horticulturae 713: VI International Peach Symposium
CYTOCHEMICAL ANALYSIS OF WOOLLY AND NORMAL NECTARINE MESOCARP |
| Authors: | M.E. Rodriguez, L.A. Lizana |
| Keywords: | cell walls, tissue microscopy, selective stains |
| Abstract:
Ripe nectarine fruits cv. ‘Flamekist’ (firmness 9N) presenting the physiological disorder woolliness and healthy fruits were selected for a cytochemical study of the mesocarp cells. Affected and healthy mesocarp tissue were infiltrated with glycol methacrylate resin. The Schiff’s reagent with periodic acid pre-treatment was used to show total insoluble carbohydrates. The β-1,4 glucans and pectin were located using Calcofluor white MR2 and Coriphosphine, respectively. The healthy as well as the woolly fruit showed intercellular spaces in the parenchyma tissue, due to the separation of adjacent cells. There was no difference in the cell walls (cellulose fraction) of both woolly and healthy cells, since both presented intense staining, while intercellular spaces did not stain. Samples stained with Coriphosphine showed marked differences between woolly and healthy tissue. In both tissues cell walls appear clearly outlined, but in woolly tissue the intensity of the fluorescence was higher. Healthy tissue did not show stain in the intercellular spaces, while in the woolly tissue many of the intercellular spaces appeared intensely fluorescent. This indicates the accumulation of pectic substances in the cell walls and in the intercellular spaces in woolly tissue. | |
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