ISHS Acta Horticulturae 712: IV International Conference on Managing Quality in Chains - The Integrated View on Fruits and Vegetables Quality
REGULATION OF O2 LEVELS IN CONTROLLED ATMOSPHERE STORAGE ON THE RESPIRATORY AND ACTIVITY OF TCA CYCLE RELATES ENZYMES IN CUCUMBER FRUIT |
| Authors: | V. Srialong, Y. Tatsumi, M. Ishimaru |
| Keywords: | Respiration, cucumber, succinate dehydrogenase, shdB gene |
| Abstract:
Respiratory pattern and succinate dehydrogenase (SDH) activity in cucumber fruit stored in 5%, 21% (air control) and 100% O2 at 5°C were studied. Storage in 100% O2 at 5°C was most effective for suppressing the respiratory rate in cucumber fruit, the rate being lower than in fruit stored in either 5% O2 or air. Relative activity of SDH in cucumber fruit stored in 100% O2 was lower than in other storage conditions. Only slightly different SDH activity was recorded between control and 5% O2-treated fruit. Northern hybridization with a partial cDNA of cucumber sdhB as a probe revealed a constant level of shdB gene expression during storage. These results suggesting that change of SDH is post-transcriptionally regulated. | |
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