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ISHS Acta Horticulturae 708: V International Strawberry Symposium

OPTIMIZATION OF A LIQUID MEDIUM FOR GERMINATION OF STRAWBERRY POLLEN

Authors:   M. Ariza, J.M. Lopez-Aranda, C. Soria
Keywords:   Fragaria × ananassa, cultivars, pollen germination, boron, sucrose
Abstract:
Misshaped fruit is an important problem in strawberry production in the Huelva area (South-western coast Spain). Physiological and breeding studies on this topic require a deeper knowledge of factors affecting the fertilization process, such as pollen germination. To develop a simple and suitable medium to improve in vitro pollen germination of strawberry cultivars, liquid media containing different combinations of boron (from 0 to 350 ppm) and sucrose (5 to 25%) were tested. Percentages of pollen germination ranged from 0.5 to 56 %. The higher values were obtained on media containing 10-20 % sucrose and 100-350 ppm boric acid.

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