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| Authors: | S. Monticelli, M. Meneghini, C. Damiano, G. Baruzzi, V. Kondakova, B. Watillon |
| Keywords: | Fragaria × ananassa, F. vesca, gene escape, genetic transformation, GUS expression, pollination |
Abstract:
The escape of transgenes in pollen spread from GM crops, particularly in the presence of wild relatives, is of increasing concern.
To investigate the horizontal transmission of transgenes, strawberry has been chosen among the perennial species.
The availability of in vitro regeneration systems from leaves, stipules, and roots has allowed the comparison of their potential efficiency in three strawberry genotypes, and the detection of suitable targets for genetic manipulation.
The GUS marker gene was used to evaluate transgene expression under in vitro and in vivo conditions, and to provide information about its transmission to intra- and inter-specific progeny.
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