ISHS Acta Horticulturae 708: V International Strawberry Symposium
DIFFERENTIAL EXPRESSED GENOME FRACTIONS AND TRANSCRIPTIONAL FACTOR DOMAIN ISOLATION IN FRAGARIA SPP. |
| Authors: | L. Milella, M. Lapelosa, I. Greco, G. Martelli |
| Keywords: | Strawberry, ripening investigation, differential display, epimerase |
| Abstract:
In recent years, molecular and genetic analysis of fruit development, especially ripening of fleshy fruit, has resulted in significant gains in knowledge of metabolic pathways involved in commercial and nutritional fruit qualities. Ripening has an impact on fibre content and composition, lipid metabolism, levels of vitamins and various antioxidant compounds. The ability to understand and to manipulate through breeding or biotechnology, key control points and regulatory points of specific pathways involved in the ripening process such as carotenoids, flavonoids, vitamins, and flavour volatiles biosynthesis, will allow the control of nutritional and quality characteristics linked to ripening. The present work aimed to isolate DNA fractions and transcriptional factor domains differentially expressed during fruit ripening in Fragaria spp. Fruit from 10 different genotypes were collected at three different ripening stages from green to ripe. Total RNAs were isolated from fruit tissue, and cDNAs were obtained in order to perform several experiments to isolate differentially expressed fractions. Random and specific primers were utilized in order to isolate sequences of genes involved in the ripening process. All fragments differentially expressed among the ripening stages and from the different genotypes were sequenced. The sequences obtained were compared to databases to evaluate similarity with genes already isolated. Sequences showed high homology with genes encoding protein with known activity such as epimerase, carotene desaturase and zinc metabolism enzymes etc. At the same time, several transcriptional factors were found, in particular some that are involved in sugar metabolism. All fragments isolated were used to produce a specific a cDNA library. | |
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