ISHS Acta Horticulturae 708: V International Strawberry Symposium
PATHOGENICITY, MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF RHIZOCTONIA SPP. ISOLATES FROM STRAWBERRY PLANTS IN ISRAEL |
| Authors: | M. Sharon, B. Sneh, S. Freeman |
| Keywords: | Rhizoctonia, Fragaria x ananassa, pathogenicity, rDNA-ITS |
| Abstract:
Methyl-bromide is currently used as a soil fumigant for strawberry. However, its use is restricted internationally and in Israel from 2005. Thus, soil borne patho-gens including Rhizoctonia spp., may cause more serious losses than previously re-ported. It is therefore important to study Rhizoctonia populations on strawberry in Israel. Anastomosis grouping (AG), the method currently used to classify Rhizoctonia isolates, is accurate and widely used; however, it is time and labour consuming. In addition, certain isolates do not self anastomose, or anastomose with more than one group. In the present work, rDNA-ITS sequence multiple alignment was used to clas-sify the isolates. The isolates were consistently clustered according to their AGs. Cur-rently, determination of pathogenicity of Rhizoctonia spp. isolates on strawberry is based on reduction of plant biomass rather than on specific disease symptoms. The duration of this test is relatively time-consuming (3-5 weeks). Based on inoculation of strawberry petioles, fruit and young seedlings derived from strawberry seeds, inocu-lation of seedlings at the two-true-leaf stage was found to be an adequate method for determining pathogenicity of Rhizoctonia isolates, and is less time-consuming than the method currently used. | |
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