ISHS Acta Horticulturae 706: IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop
TOWARD UNDERSTANDING BRASSICA RAPA SSP. PEKINENSIS GENOME AND ITS EVOLUTION THROUGH BES ANALYSIS |
| Authors: | S.R. Choi, C.P. Hong, D.H. Koo, P. Plaha, T.J. Yang, B.S. Park, J.W. Bang, Y.P. Lim |
| Keywords: | Brassica rapa ssp. pekinensis, BES, protein-coding regions, transposable elements, tandem satellite DNAs |
| Abstract:
We generated 1,812 BAC-end sequences (BESs) from 1,473 BAC clones, with an average length of 354 bases, to understand the genome structure of Brassica rapa ssp. pekinensis. Analysis using BLAST and motif searches revealed that 35.5%, 12.3%, 1.8%, 23.2% of the BESs contained protein-coding regions, transposable elements, microsatellites and tandem satellite DNAs, respectively. A comparison of the functional categories of protein-coding regions with those of Arabidopsis thaliana revealed nearly the similar distribution in both the species. However, proteins pertaining to metabolism, development and sub-cellular localization were comparatively higher in A. thaliana. Transposable elements, especially retrotransposons, were more abundant in B. rapa than in A. thaliana. It is suggested that, in addition to triplication, retrotranposons are involved in the genome expansion of B. rapa. The BES analysis is a useful tool to elucidate the features of an uncharacterized and complex genome of B. rapa. | |
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